Transforming growth factorβ (TGB-β) is a potent modulator of proliferation and differentiation in bony tissue, and might have a role in the control of bone formation and remodeling. Monitoring these pattern of genes and protein expression
during
tumor
development will provide a basis for understanding normal growth and distortion of osteochondrogenesis.
The expression and distribution of TGF-βwas studied in 34 bone tumors that consisted of odontogenic myxoma 3 cases, osteoma 5 cases, exostosis 4 cases, chondrosarcoma 3 cases, osteosarcoma 4 cases, fibrous dysplasia 4 cases of mandible
cementossifying
fibroma 4 cases, ossifying fibroma 4 cases, malignant fibrous histiocytoma 3 cases. And normal bone was used for standardization of the expression and distribution of TGF-β. In order to analyze the expression of TGF-β in the tumor types,
immunohistochemical stain was performed with TGF-β as a primary antibody and anti-mouse IgG as a secondary antibody.
Results about interrelationship between TGF-βexpression and bone tumor as follows with immunohistochemical stain for TGF-β: TGF-βexpression was mild in osteoblast of normal bone and negative in bone matrix, TGF-βexpression in osteoma was more
increased than normal bone and exostosis. TGF-βexpression was negative in fibroblast of cementifying fibroma and ossifying fibroma. TGF-βexpression was moderate in cementoblast and osteoblast and cementocyte or osteocyte of cementifying fibroma
and
ossifying fibroma. TGF-βwas the most expression fibrous dysplasia but especially woven bone and fibroblasts in interlacing collagen fiber. Especially, it seems that strong of TGF-βin fibrous dysplasia due to coupling effect of bone resoprtion
and
subsequent bone formation. TGF-β expression was negative in fibroblast and histiocyte of malignant fibrous histiocytoma. TGF-βexpression was negative in inner fibrous tissue of osteosarcoma, moderate in osteoid of osteosarcoma and mild in
atypical
osteoblast. TGF-βexpression was severe in nuclear and cytoplasm of atypical cartilage cell and cartilaginous cell matrix. of chondrosarcoma, and negative in fibrous intercellular tissue of osteosarcoma.
In summary, the expression of TGF-β in the bone tumor is more than that of normal bone. And it has a difference between benign and malignant tumors. It should be consided that TGF-βis one of prognostic factors in bone tumor.
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